Journal: Frontiers in Endocrinology

Article Title: Ultrasound combined with microbubbles promotes diabetic wound healing by regulating macrophage polarization

doi: 10.3389/fendo.2026.1781906

Figure Lengend Snippet: Immunofluorescence staining of diabetic wound healing tissues and RT-qPCR analysis of mRNA relative expression of macrophage-related inflammatory factors. (A-F) Immunofluorescence staining images of healed wound tissues in diabetic rats: CD206 is shown in green, CD86 in red, and nuclei in blue; (G, H) Statistical graphs of immunofluorescence staining results from the three groups; (I) Statistical graph of inflammatory factor mRNA expression in RAW264.7 macrophages after 24-hour intervention; (J-L) Statistical graphs of inflammatory factor mRNA expression in wound tissues of the three groups of diabetic rats at three time points. Scale bar = 50μm; Data presented as Mean ± SD, n = 4; *p < 0.05, **p< 0.01, ***p < 0.001.

Article Snippet: After 4 μm paraffin sections were subjected to dewaxing, antigen retrieval, and BSA blocking, they were incubated overnight at 4 °C with primary antibodies against CD206 (1:200, Proteintech) and CD86 (1:50, Proteintech).

Techniques: Immunofluorescence, Staining, Quantitative RT-PCR, Expressing